Enzymatic assay for conjugated bilirubin (Bc) in serum using bilirubin oxidase (BOD)

Determination of conjugated and total bilirubin in serum of neonates, with use of bilirubin oxidase.

A new, simple, and rapid assay for conjugated bilirubin that does not require serum-matrix standards was developed by using the enzyme bilirubin oxidase (EC 1.3.3.5). This procedure can also be modified to measure total bilirubin. Measurements from these assays were compared with results obtained with the Sigma 605 (Jendrassik-Gróf method), Sigma 550/551 (Walters-Gerarde method), and Kodak Ekta...

Comparing transcutaneous bilirubin versus serum bilirubin measurement using spectrophotometry for jaundice screening of full-term neonates

Background: Neonatal jaundice is an almost common problem among neonates that may cause complications such as kernicterus in severe cases. Since early diagnosis and proper treatment of neonatal jaundice is vital to prevent the related complications, it is necessary to check bilirubin before discharging the neonates. There are several evaluating and diagnostic methods to measure the level of bil...

Paraprotein interference in an assay of conjugated bilirubin.

References 1. Lo YMD, Hjelm NM, Fidler C, Sargent IL, Murphy MF, Chamberlain PF, et al. Prenatal diagnosis of fetal RhD status by molecular analysis of maternal plasma. N Engl J Med 1998;339:1734–8. 2. Chiu RWK, Lau TK, Leung TN, Chow KCK, Chui DHK, Lo YMD. Prenatal exclusion of thalassaemia major by examination of maternal plasma. Lancet 2002;360:998–1000. 3. Zhong XY, Laivuori H, Livingston J...

Does conjugated bilirubin displace unconjugated bilirubin from albumin.

Measurement of total bilirubin by use of bilirubin oxidase.

We used an enzymatic method for measuring total bilirubin in serum. Results by this method varied linearly with bilirubin concentrations to at least 300 mg/L. The day-to-day precision (CV) of the method ranged from less than 1% to about 11% at bilirubin concentrations of 183 and 12 mg/L, respectively. Commonly used anticoagulants, serum preparation materials, and selected drugs had no effect on...